Current Review of Genetically Modified Lactic Acid Bacteria for the Prevention and Treatment of Colitis Using Murine Models

Alejandra de Moreno de Leblanc (1) , Silvina del Carmen (1) , Jean-Marc J.-M. Chatel (2) , Anderson Miyoshi (3) , Vasco Azevedo (3) , Philippe P. Langella (2) , Luis L. Bermudez Humaran (2) , Jean Guy Leblanc (1)

1 CONICET - Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires]
2 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
3 UFMG - Universidade Federal de Minas Gerais = Federal University of Minas Gerais [Belo Horizonte, Brazil]

Inflammatory Bowel Diseases (IBD) are disorders of the gastrointestinal tract characterized by recurrent inflammation that requires lifelong treatments. Probiotic microorganisms appear as an alternative for these patients; however, probiotic characteristics are strain dependent and each probiotic needs to be tested to understand the underlining mechanisms involved in their beneficial properties. Genetic modification of lactic acid bacteria (LAB) was also described as a tool for new IBD treatments. The first part of this review shows different genetically modified L(GM-LAB) described for IBD treatment since 2000. Then, the two principally studied strategies are discussed (i) GM-Lproducing antioxidant enzymes and (ii) GM-Lproducing the anti-inflammatory cytokine IL-10. Different delivery systems, including protein delivery and DNA delivery, will also be discussed. Studies show the efficacy of GM-L(using different expression systems) for the prevention and treatment of IBD, highlighting the importance of the bacterial strain selection (with anti-inflammatory innate properties) as a promising alternative. These microorganisms could be used in the near future for the development of therapeutic products with anti-inflammatory properties that can improve the quality of life of IBD patients.

Domaines: Sciences du Vivant [q-bio]

Alejandra de Moreno de Leblanc, Silvina del Carmen, Jean-Marc J.-M. Chatel, Anderson Miyoshi, Vasco Azevedo, et al.. Current Review of Genetically Modified Lactic Acid Bacteria for the Prevention and Treatment of Colitis Using Murine Models. Gastroenterology research and practice, 2015, pp.1-8. ⟨10.1155/2015/146972⟩. ⟨hal-02637902⟩