Progressive adaptation of a CpGV isolate to codling moth populations resistant to CpGV-M

Benoit Graillot (1, 2) , Marie Berling (1) , Christine Blachère-Lopez (3, 1) , Myriam Siegwart (4) , Samantha Besse (2) , Miguel Lopez-Ferber (1)

1 LGEI - Laboratoire de Génie de l'Environnement Industriel
2 Natural Plant Protection
3 DPT SPE - Département Santé des Plantes et Environnement
4 PSH - Unité de recherche Plantes et Systèmes de Culture Horticoles

The NPP-R1 isolate of CpGV is able to replicate on CpGV-M-resistant codling moths. However, its efficacy is not sufficient to provide acceptable levels of control in natural (orchard) conditions. A laboratory colony derived from resistant codling moths was established, which exhibited a homogeneous genetic background and a resistance level more than 7000 fold. By successive cycles of replication of NPP-R1 in this colony, we observed a progressive increase in efficacy. After 16 cycles (isolate 2016-r16), the efficacy of the virus isolate was equivalent to that of CpGV-M on susceptible insects. This isolate was able to control both CpGV-M-susceptible and CpGV-M-resistant insects with similar efficacy. No reduction in the levels of occlusion body production in susceptible larvae was observed for 2016-r16 compared to CpGV-M.

Keywords: Cydia pomonella granulovirus; resistance development; coddling moth; biological control

Domains: Life Sciences [q-bio]; Environmental Sciences

Benoit Graillot, Marie Berling, Christine Blachère-Lopez, Myriam Siegwart, Samantha Besse, et al.. Progressive adaptation of a CpGV isolate to codling moth populations resistant to CpGV-M. Viruses, 2014, 6 (12), pp.5135-5144. ⟨10.3390/v6125135⟩. ⟨hal-02635330⟩