Genome-wide analysis in Hevea brasiliensis laticifers revealed species-specific post-transcriptional regulations of several redox-related genes

Yi Zhang (1) , Julie Leclercq (1, 2) , Shuangyang Wu (1, 3) , Enrique Ortega-Abboud (1) , Stéphanie Pointet (1) , Chaorong Tang (4) , Songnian Hu (5) , Pascal Montoro (1, 2)

1 UMR AGAP - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales
2 Cirad-BIOS - Département Systèmes Biologiques
3 UCAS - University of Chinese Academy of Sciences [Beijing]
4 CATAS - Chinese Academy of Tropical Agricultural Sciences
5 CAS - Chinese Academy of Sciences

MicroRNA-mediated post-transcriptional regulation has been reported on ROS production and scavenging systems. Although microRNAs first appeared highly conserved among plant species, several aspects of biogenesis, function and evolution of microRNAs were shown to differ. High throughput transcriptome and degradome analyses enable to identify small RNAs and their mRNA targets. A non-photosynthetic tissue particularly prone to redox reactions, laticifers from Hevea brasiliensis, revealed species-specific post-transcriptional regulations. This paper sets out to identify the 407 genes of the thirty main redox-related gene families harboured by the Hevea genome. There are 161 redox-related genes expressed in latex. Thirteen of these redox-related genes were targeted by 11 microRNAs. To our knowledge, this is the first report on a mutation in the miR398 binding site of the cytosolic CuZnSOD. A working model was proposed for transcriptional and post-transcriptional regulation with respect to the predicted subcellular localization of deduced proteins.

Domaines: Biologie végétale

Yi Zhang, Julie Leclercq, Shuangyang Wu, Enrique Ortega-Abboud, Stéphanie Pointet, et al.. Genome-wide analysis in Hevea brasiliensis laticifers revealed species-specific post-transcriptional regulations of several redox-related genes. Scientific Reports, 2019, 9, ⟨10.1038/s41598-019-42197-8⟩. ⟨hal-02618920⟩