Spray-drying microencapsulation of nisin by complexation with exopolysaccharides produced by probiotic Bacillus tequilensis-GM and Leuconostoc citreum-BMS

Yousra Abid (1) , Adem Gharsallaoui (2) , Emilie Dumas (2) , Sami Ghnimi (2) , Hamadi Attia (1) , Samia Azabou (1)

1 LAVASA - Laboratoire Analyses, Valorisation et Sécurité des Aliments, Ecole Nationale d’Ingénieurs de Sfax
2 BIODYMIA - Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires

This work aims to encapsulate nisin by complexation with exopolysaccharides (EPS), produced by Bacillus tequilensis-GM and Leuconostoc citreum-BMS, namely EPS-GM and EPS-BMS, respectively, using spray-drying technique, and to evaluate the effect of this encapsulation on the structure of nisin. Results related to suspensions turbidity showed that EPS/nisin complexes were formed through electrostatic attractions. These interactions were confirmed by Fourier transform infrared (FTIR) analysis. Scanning electron microscopy (SEM) micrographs of the spray-dried complexes revealed the presence of well-separated spherical microcapsules. Besides, results obtained by UV spectra showed that no significant changes occurred on EPS-GM/nisin microcapsules suggesting that this EPS may act as protective agent of nisin structure against spray-drying conditions.

Mots clés: Nisin; Microencapsulation; Exopolysaccharides; Spray-drying

Domaines: Génie des procédés; Alimentation et Nutrition; Ingénierie des aliments

Yousra Abid, Adem Gharsallaoui, Emilie Dumas, Sami Ghnimi, Hamadi Attia, et al.. Spray-drying microencapsulation of nisin by complexation with exopolysaccharides produced by probiotic Bacillus tequilensis-GM and Leuconostoc citreum-BMS. Colloids and Surfaces B: Biointerfaces, 2019, 181, pp.25-30. ⟨10.1016/j.colsurfb.2019.05.022⟩. ⟨hal-02495852⟩