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Journal articles
KAP1 is an antiparallel dimer with a functional asymmetry
Giulia Fonti
1
Maria Marcaida
1
Louise Bryan
2
Sylvain Träger
1
Alexandra Kalantzi
1
Pierre-Yves Jl Helleboid
2
Davide Demurtas
2
Mark Tully
3
Sergei Grudinin
4
Didier Trono
5
Beat Fierz
2
Matteo Dal Peraro
1
4
NANO-D - Algorithms for Modeling and Simulation of Nanosystems
Inria Grenoble - Rhône-Alpes, Grenoble INP - Institut polytechnique de Grenoble - Grenoble Institute of Technology, LJK - Laboratoire Jean Kuntzmann
Alexandra Kalantzi 1
Author
Pierre-Yves Jl Helleboid 2
Author
Davide Demurtas 2
Author
Mark Tully 3
Author
Sergei Grudinin 4
Author Employer institution : Centre National de la Recherche Scientifique IdHAL : sergei-grudinin ORCID : https://orcid.org/0000-0002-1903-7220
Didier Trono 5
Author
Beat Fierz 2
Author
Matteo Dal Peraro 1
Author
1
SIB - Swiss Institute of Bioinformatics [Lausanne] (Quartier UNIL-Sorge Bâtiment Génopode CH-1015 Lausanne - Switzerland)
- UNIL - Université de Lausanne (CH-1015 Lausanne - Switzerland)
2
EPFL - Ecole Polytechnique Fédérale de Lausanne (CH-1015 Lausanne, Switzerland - Switzerland)
3
ESRF - European Synchrotron Radiation Facility (6 rue Jules Horowitz BP220 38043 GRENOBLE CEDEX - France)
4
NANO-D - Algorithms for Modeling and Simulation of Nanosystems (Inria GIANT DRT/LETI/DACLE Batiment 51C - Minatec Campus 17 rue des Martyrs 38054 Grenoble Cedex - France)
- Inria Grenoble - Rhône-Alpes (Inovallée
655 avenue de l'Europe
38330 Montbonnot - France)
- Inria - Institut National de Recherche en Informatique et en Automatique (Domaine de Voluceau Rocquencourt - BP 105 78153 Le Chesnay Cedex - France)
- Grenoble INP - Institut polytechnique de Grenoble - Grenoble Institute of Technology (46 avenue Félix Viallet 38031 Grenoble Cedex 1 - France)
- LJK - Laboratoire Jean Kuntzmann (Bâtiment IMAG, CS 40700, F-38058 Grenoble Cedex 9 - France)
- Grenoble INP - Institut polytechnique de Grenoble - Grenoble Institute of Technology (46 avenue Félix Viallet 38031 Grenoble Cedex 1 - France)
- Inria - Institut National de Recherche en Informatique et en Automatique (Domaine de Voluceau Rocquencourt - BP 105 78153 Le Chesnay Cedex - France)
- CNRS - Centre National de la Recherche Scientifique (France)
- UGA [2016-2019] - Université Grenoble Alpes [2016-2019] (CS 40700 - 38058 Grenoble cedex - France)
5
School of Life Sciences [Lausanne] (Lausanne - Switzerland)
- EPFL - Ecole Polytechnique Fédérale de Lausanne (CH-1015 Lausanne, Switzerland - Switzerland)
Abstract : KAP1 (KRAB-domain associated protein 1) plays a fundamental role in regulating gene expression in mammalian cells by recruiting different transcription factors and altering the chromatin state. In doing so, KAP1 acts both as a platform for macromolecular interactions and as an E3 SUMO ligase. This work sheds light on the overall organization of the full-length protein combining solution scattering diffraction data, integrative modeling and single-molecule experiments. We show that KAP1 is an elongated antiparallel dimer with a native asymmetry at the C-terminal domain. This conformation supports our finding that the RING domain contributes to KAP1 auto-SUMOylation. Importantly, this intrinsic asymmetry has key functional implications for the KAP1 network of interactions, as the heterochromatin protein 1 (HP1) occupies only one of the two putative HP1 binding sites on the KAP1 dimer, resulting in an unexpected stoichiometry, even in the context of chromatin fibers.
Document type :
Journal articles
Complete list of metadatas
https://hal.archives-ouvertes.fr/hal-02291553
Contributor : Sergei Grudinin <>
Submitted on : Thursday, September 19, 2019 - 4:54:27 AM
Last modification on : Tuesday, November 24, 2020 - 5:04:04 PM
Contributor : Sergei Grudinin <>
Submitted on : Thursday, September 19, 2019 - 4:54:27 AM
Last modification on : Tuesday, November 24, 2020 - 5:04:04 PM
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Identifiers
- HAL Id : hal-02291553, version 1
- DOI : 10.26508/lsa.201900349
Citation
Giulia Fonti, Maria Marcaida, Louise Bryan, Sylvain Träger, Alexandra Kalantzi, et al.. KAP1 is an antiparallel dimer with a functional asymmetry. Life Science Alliance, Life Science Alliance LLC, 2019, 2 (4), pp.e201900349. ⟨10.26508/lsa.201900349⟩. ⟨hal-02291553⟩
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