Tracking plasma generated H2O2 from gas into liquid phase and revealing its dominant impact on human skin cells

Joern Winter (1, 2) , H. Tresp (1, 2) , M U Hammer (2, 1) , S. Iseni (2, 1) , S. Kupsch (1, 2) , A. Schmidt-Bleker (1, 2) , K. Wende (2, 1) , M. Dünnbier (1, 2) , K. Masur (1, 2) , K.-D. Weltmann (2) , S. Reuter (1, 2)

1 The center for innovation competence (ZIK) plasmatis
2 INP - Leibniz Institute for Plasma Science and Technology

The pathway of the biologically active molecule hydrogen peroxide (H 2O2) from the plasma generation in the gas phase by an atmospheric pressure argon plasma jet, to its transition into the liquid phase and finally to its inhibiting effect on human skin cells is investigated for different feed gas humidity settings. Gas phase diagnostics like Fourier transformed infrared spectroscopy and laser induced fluorescence spectroscopy on hydroxyl radicals (•OH) are combined with liquid analytics such as chemical assays and electron paramagnetic resonance spectroscopy. Furthermore, the viability of human skin cells is measured by Alamar Bluel assay. By comparing the gas phase results with chemical simulations in the far field, H2O2 generation and destruction processes are clearly identified. The net production rate of H2O2 in the gas phase is almost identical to the H2O2 net production rate in the liquid phase. Moreover, by mimicking the H2O2 generation of the plasma jet with the help of an H2O2 bubbler it is concluded that the solubility of gas phase H2O 2 plays a major role in generating hydrogen peroxide in the liquid. Furthermore, it is shown that H2O2 concentration correlates remarkably well with the cell viability. Other species in the liquid like •OH or superoxide anion radical do not vary significantly with feed gas humidity. © 2014 IOP Publishing Ltd.

Mots clés: [gas phase; human skin cells; humidity; hydrogen p

Domaines: Physique des plasmas [physics.plasm-ph]; Plasmas; Optique / photonique; Milieux fluides et réactifs; Chimie-Physique [physics.chem-ph]; Ingénierie biomédicale

Joern Winter, H. Tresp, M U Hammer, S. Iseni, S. Kupsch, et al.. Tracking plasma generated H2O2 from gas into liquid phase and revealing its dominant impact on human skin cells. Journal of Physics D: Applied Physics, 2014, 47 (28), pp.285401--285401. ⟨10.1088/0022-3727/47/28/285401⟩. ⟨hal-02270229⟩