A chymotryptic-type serine protease is required for IL-2 production by Jurkat T cells.

Abstract : Interleukin-2 (IL-2) production by activated Jurkat T cells was markedly delayed when these cells were treated with low concentrations of the chymotryptic-type protease inhibitor N-alpha-p-tosyl-L-phenylalanine chloromethylketone (TPCK). This increased lag time observed in the presence of TPCK directly correlates with the interaction of the inhibitor with a unique 42,000 molecular weight (MW) serine protease, which can be labelled with [3H]DFP, and was not due to an intracellular accumulation of a non-mature form of IL-2 nor to a non-specific inhibition of overall protein synthesis. The results presented in this report indicate that a 42,000 MW chymotryptic-like serine protease is required for IL-2 production by activated Jurkat T cells.
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https://hal.archives-ouvertes.fr/hal-02266495
Contributor : Bernard Mari <>
Submitted on : Wednesday, August 14, 2019 - 4:09:55 PM
Last modification on : Wednesday, August 14, 2019 - 4:09:55 PM

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  • HAL Id : hal-02266495, version 1
  • PUBMED : 2394468

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P Auberger, S Sonthonnax, Jf Peyron, B Mari, M Fehlmann. A chymotryptic-type serine protease is required for IL-2 production by Jurkat T cells.. Immunology, Wiley, 1990, 70 (4), pp.547-50. ⟨hal-02266495⟩

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