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Poster communications

Using GC-MS and helium to resolve positional isomers of trans-C16:1 and trans- C18:1 fatty acids

Abstract : Separation and analysis of positional isomers of trans-C16:1 and trans-C18:1 fatty acids usually rely on gas chromatography (GC), with flame-ionization detector (FID) and hydrogen as gas vector. Because hydrogen results in good peak resolution, it is unclear whether the use of mass spectroscopy (MS) and helium as gas vector would yield worse outcomes. Therefore, this study aimed at showing the abilities of GC-MS when dealing with positional isomers of trans-C16:1 and trans-C18:1 fatty acids. GC-MS in combination with helium was used. Both partially hydrogenated fish oil (PHFO) and dairy products, derivatized as fatty acid methyl esters (FAME), were considered because of differences of trans-C16:1 and trans-C18:1 isomer distribution. To rule out the effect of stationary phase, two different GC columns were used. Optimized conditions determined in each case. Using optimized conditions, GC-MS and helium led to decent resolution of trans-C16:1 and trans-C18:1 positional isomers, in respect with what can be obtained with hydrogen and FID. This held true for both dairy products and PHFO for which differences in profiles were clearly highlighted. Both GC columns provided similar resolutions. GC-MS in combination with helium is suitable for analysis of positional isomers of trans-C16:1 and trans-C18:1 fatty acids.
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Poster communications
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https://hal.archives-ouvertes.fr/hal-02177903
Contributor : Etienne Guillocheau Connect in order to contact the contributor
Submitted on : Tuesday, July 9, 2019 - 1:49:27 PM
Last modification on : Wednesday, April 6, 2022 - 4:08:21 PM

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Étienne Guillocheau, Daniel Catheline, Philippe Legrand, Vincent Rioux. Using GC-MS and helium to resolve positional isomers of trans-C16:1 and trans- C18:1 fatty acids. 2018 AOCS Annual Meeting & Expo, May 2018, Minneapolis, United States. 2018, ⟨10.13140/RG.2.2.20921.47209⟩. ⟨hal-02177903⟩

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