Oral immunization with a synthetic peptide of cholera toxin B subunit. Obtention of neutralizing antibodies.

Abstract : The ability of free synthetic fragments of the cholera toxin (CT), administered by parenteral or oral route, without adjuvant, to induce antibodies cross-reacting with CT was tested. Two peptides corresponding to the sequences 30-50 and 50-75 of the CT beta chain were selected and synthesized. Both free peptides, given intraperitoneally or orally, without adjuvant, elicited seric antibodies cross-reacting with CT. The anti-(P50-75) antibodies were able to neutralize the CT activity. Our results show that protection against a toxin at the systemic level can be obtained with a synthetic peptide even when administered by an oral route.
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https://hal.archives-ouvertes.fr/hal-02146964
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Submitted on : Tuesday, June 4, 2019 - 12:08:14 PM
Last modification on : Tuesday, June 4, 2019 - 4:30:06 PM

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  • HAL Id : hal-02146964, version 1
  • PUBMED : 3758076

Citation

A Guyon-Gruaz, A Delmas, S Pedoussaut, H Halimi, G Milhaud, et al.. Oral immunization with a synthetic peptide of cholera toxin B subunit. Obtention of neutralizing antibodies.. European Journal of Biochemistry, Wiley, 1986, 159 (3), pp.525-8. ⟨hal-02146964⟩

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