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A micromethod for quantitative determination of iodoamino acids in thyroglobulin.

Abstract : We describe a new method for quantification of iodoamino acids after enzymatic hydrolysis of thyroglobulin. The procedure involves separation of monoiodotyrosine (MIT), di-iodotyrosine, tri-iodothyronine and thyroxine by reverse phase HPLC with a Vydac C18 stationary phase and a mobile phase of water-acetonitrile-acetic acid. The separation is monitored by sensitive spectrophotometric detection through a 96-well microplate system based on the catalytic Sandell-Kolthoff reaction of iodide on the oxidation of arsenic(III) by cerium(IV). This new microassay is particularly convenient because of its high sensitivity and its rapidity (less than 2 h). It can detect 1 pmol MIT and 0.5 pmol of the other three iodoamino acids with a recovery higher than 96%. Moreover, the 96-well microplate system allows many samples to be tested simultaneously and avoids the use of radiolabeled iodine.
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Journal articles
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https://hal.archives-ouvertes.fr/hal-02146142
Contributor : Jean-Louis Franc <>
Submitted on : Monday, June 3, 2019 - 4:03:55 PM
Last modification on : Monday, June 3, 2019 - 4:03:55 PM

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  • HAL Id : hal-02146142, version 1
  • PUBMED : 9135574

Citation

Nathalie Baudry, B Mallet, J. Lejeune, L. Vinet, Jean-Louis Franc. A micromethod for quantitative determination of iodoamino acids in thyroglobulin.. Journal of Endocrinology, BioScientifica, 1997, 153 (1), pp.99-104. ⟨hal-02146142⟩

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