Development of antifungal ingredients for dairy products: From in vitro screening to pilot scale application

Lucille Garnier (1) , Jérôme Mounier , Sébastien Lê (2) , Audrey Pawtowski , Nicolas Pinon (1) , Benedicte Camier (1) , Manon Chatel (1) , Gilles Garric (1) , Anne Thierry (1) , Emmanuel Coton , Florence Valence (1)

1 STLO - Science et Technologie du Lait et de l'Oeuf
2 Mathématiques Appliquées

Biopreservation represents a complementary approach to traditional hurdle technologies for reducing microbialcontaminants (pathogens and spoilers) in food. In the dairy industry that is concerned by fungal spoilage,biopreservation can also be an alternative to preservatives currently used (e.g. natamycin, potassium sorbate).The aim of this study was to develop antifungal fermentates derived from two dairy substrates using a sequential approach including an in vitro screening followed by an in situ validation. The in vitro screening of the antifungal activity of fermentates derivating from 430 lactic acid bacteria (LAB) (23 species), 70 propionibacteria (4 species) and 198 fungi (87 species) was performed against four major spoilage fungi (Penicillium commune, Mucor racemosus, Galactomyces geotrichum and Yarrowia lipolytica) using a cheese-mimicking model. The most active fermentates were obtained from Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus casei/paracasei and Lactobacillus plantarum among the tested LAB, Propionibacterium jensenii among propionibacteria, and Mucor lanceolatus among the tested fungi. Then, for the 11 most active fermentates, culture conditions were optimized by varying incubation time and temperature in order to enhance their antifungal activity. Finally, the antifungal activity of 3 fermentates of interest obtained from Lactobacillus rhamnosus CIRM-BIA1952, P. jensenii CIRMBIA1774 and M. lanceolatus UBOCC-A-109193 were evaluated in real dairy products (sour cream and semi-hard cheese) at a pilot-scale using challenge and durability tests. In parallel, the impact of these ingredients on organoleptic properties of the obtained products was also assessed. In semi-hard cheese, application of the selected fermentates on the cheese surface delayed the growth of spoilage molds for up to 21 days, without any effect on organoleptic properties, P. jensenii CIRM-BIA1774 fermentate being the most active. In sour cream, incorporation of the latter fermentate at 2 or 5% yielded a high antifungal activity but was detrimental to the product organoleptic properties. Determination of the concentration limit, compatible with product acceptability, showed that incorporation of this fermentate at 0.4% prevented growth of fungal contaminants in durability tests but had a more limited effect against M. racemosus and P. commune in challenge tests. To our knowledge, this is the first time that the workflow followed in this study, from in vitro screening using dairy matrix to scale-up in cheese and sour cream, is applied for production of natural ingredients relying on a large microbial diversity in terms of species and strains. This approach allowed obtaining several antifungal fermentates which are promising candidates for dairy products biopreservation.

Mots clés: lactic acid bacteria; dairy product; biopréservation; champignon; cryopreservation; food preservation

Mots clés: agent antifongique; conservation biologique; screening; conservation des aliments; produit laitier; bactérie lactique; propionibacteria; conservateur naturel; contamination fongique

Domaines: Microbiologie et Parasitologie; Alimentation et Nutrition

Lucille Garnier, Jérôme Mounier, Sébastien Lê, Audrey Pawtowski, Nicolas Pinon, et al.. Development of antifungal ingredients for dairy products: From in vitro screening to pilot scale application. Food Microbiology, 2019, 81, pp.97-107. ⟨10.1016/j.fm.2018.11.003⟩. ⟨hal-01998876⟩