ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems

Veronica Sorgato (1) , Michel Berger (1) , Charlotte Emain (1) , Christine Vever-Bizet (2) , Jean-Marc Dinten (3) , Geneviève Bourg-Heckly (2) , Anne Planat-Chrétien (1)

1 CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information
2 SU FM - Sorbonne Université - Faculté de Médecine
3 Minatec

We have developed an adaptive calibration algorithm and protocol (ACA-Pro) that corrects from the instrumental response of various spatially resolved diffuse reflectance spectroscopy (DRSsr) systems to enable the quantification of absorption and scattering properties based on a Monte Carlo-based look-up-table approach. The protocol involves the use of a calibration reference base built with measurements of a range of different diffusive intralipid phantoms. Moreover, an advanced strategy was established to take into account the experimental variations with an additional measurement of a common solid material, allowing the use of a single calibration reference base for all experiments. The ACA-Pro is validated in contact and noncontact probe-based DRSsr systems. Furthermore, the first results of a setup replacing the probe with a CCD detector are shown to confirm the robustness of the approach.

Mots clés: spectroscopy; calibration; reflectance; optical properties; quantification

Domaines: Ingénierie biomédicale; Optique [physics.optics]

Veronica Sorgato, Michel Berger, Charlotte Emain, Christine Vever-Bizet, Jean-Marc Dinten, et al.. ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems. Journal of Biomedical Optics, 2016, 21 (6), pp.065003. ⟨hal-01973265⟩