Emergence of Nasal Carriage of ST80 and ST152 PVL+ Staphylococcus aureus Isolates from Livestock in Algeria

Amir Agabou (1, 2) , Zouleikha Ouchenane , Christelle Ngba Essebe (1) , Salim Khemissi , Mohamed Tedj Eddine Chehboub (2) , Ilyes Bey Chehboub (2) , Albert Sotto (1, 3) , Catherine Dunyach-Remy (1, 3) , Jean-Philippe Lavigne (1, 3)

1 VBMI - Virulence bactérienne et maladies infectieuses
2 UMC - Université Frères Mentouri – Constantine 1 = Constantine 1 – Frères Mentouri University
3 CHU Nîmes - Centre Hospitalier Universitaire de Nîmes

The spread of toxinogenic Staphylococcus aureus is a public health problem in Africa. The objectives of the study were to investigate the rate of S. aureus nasal carriage and molecular characteristics of these strains in livestock and humans in three Algerian provinces. Nasal samples were collected from camels, horses, cattle, sheep and monkeys, as well as humans in contact with them. S. aureus isolates were genotyped using DNA microarray. The rate of S. aureus nasal carriage varied between species: camels (53%), humans and monkeys (50%), sheep (44.2%), horses (15.2%) and cattle (15%). Nine methicillin-resistant S. aureus (MRSA) isolates (7.6%) were identified, isolated from camels and sheep. The S. aureus isolates belonged to 15 different clonal complexes. Among them, PVL+ (Panton-Valentine Leukocidin) isolates belonging to ST80-MRSA-IV and ST152-MSSA were identified in camels (n = 3, 13%) and sheep (n = 4, 21.1%). A high prevalence of toxinogenic animal strains was noted containing TSST-1- (22.2%), EDINB- (29.6%) and EtD- (11.1%) encoding genes. This study showed the dispersal of the highly human pathogenic clones ST152-MSSA and ST-80-MRSA in animals. It suggests the ability of some clones to cross the species barrier and jump between humans and several animal species.

Mots clés: Algeria; MRSA; MSSA; Panton–Valentine Leukocidin; ST152; ST80; Staphylococcus aureus; clonal complex; nasal carriage

Domaines: Toxicologie

Amir Agabou, Zouleikha Ouchenane, Christelle Ngba Essebe, Salim Khemissi, Mohamed Tedj Eddine Chehboub, et al.. Emergence of Nasal Carriage of ST80 and ST152 PVL+ Staphylococcus aureus Isolates from Livestock in Algeria. Toxins, 2017, 9 (10), ⟨10.3390/toxins9100303⟩. ⟨hal-01892113⟩