Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction

Emmanuel Scorsone (1) , Raafa Manai (1) , Maria Ricatti (2) , Marco Redaelli (2) , Philippe Bergonzo (1) , Krishna Persaud (3) , Carla Mucignat (2)

1 LCD (CEA, LIST) - Laboratoire Capteurs Diamant (CEA, LIST)
2 Unipd - Università degli Studi di Padova = University of Padua
3 School of Chemical Engineering and Analytical Sciences [Manchester]

A new bio-sensing platform based on major urinary proteins (MUPs) from the mouse as chemical recognition elements has been developed. The transducers were surface acoustic devices coated with diamond nanoparticles as an intermediate layer enabling covalent attachment of the proteins. The resulting sensors detected 2,4-Dinitrotoluene, 4-Nitrotoluene, and 2-Isobutyl-3-methoxypyrazine at ppb levels. The best sensor showed a sensitivity of 24 000 Hz . ppm(-1) to 2, 4-DNT when grafted with the protein MUP20. Trends in the sensitivity of the various VOC sensors were compared with the association constant values K-a of the proteins to target ligands measured by competitive assay in liquid phase. The system is able to detect analytes both in liquid as well as vapor phase and indicate that MUPs are robust bio-recognition elements that can be utilized in artificial olfaction applications.

Mots clés: Nanodiamond; major urinary proteins; surface acoustic wave sensors; artificial olfaction; diamond; sensor; instrumentation; nanoparticles; electronic nose

Domaines: Micro et nanotechnologies/Microélectronique; Chimie analytique; Chemo-informatique; Instrumentations et Détecteurs [physics.ins-det]

Emmanuel Scorsone, Raafa Manai, Maria Ricatti, Marco Redaelli, Philippe Bergonzo, et al.. Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction. IEEE Sensors Journal, 2016, 16 (17), pp.6543 - 6550. ⟨10.1109/JSEN.2016.2585187⟩. ⟨hal-01869478⟩