Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS

Anna Vallverdu Queralt (1, 2, 3) , Nuria Boix (2, 4) , Ester Pique (2, 4) , Jesus Gomez-Catalan (2, 4) , Alexander Medina-Remon (2, 5, 3) , Gemma Sasot (3, 5) , Merce Mercader-Marti (6) , Juan M. Llobet (2, 4) , Rosa M. Lamuela-Raventos (2, 3, 5)

1 SPO - Sciences Pour l'Oenologie
2 INSA-UB, Nutrition and Food Safety Research Institute
3 Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn)
4 GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School
5 Nutrition and Food Science Department, XaRTA, Pharmacy School
6 Miguel Torres S.A.

The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS2 and MS3 modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry

Mots clés: Red wine extract; Zebrafish (ZF); Polyphenols; Identification; Orbitrap-MS

Domaines: Sciences du Vivant [q-bio]

Anna Vallverdu Queralt, Nuria Boix, Ester Pique, Jesus Gomez-Catalan, Alexander Medina-Remon, et al.. Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS. Food Chemistry, 2015, 181, pp.146 - 151. ⟨10.1016/j.foodchem.2015.02.098⟩. ⟨hal-01837741⟩