Closed carbon balance in calculation of metabolic fluxes – Application to the central metabolism of Saccharomyces cerevisiae in wine-making fermentation
Résumé
This paper presents the metabolic-fluxes calculation of a metabolic network representing the
central metabolism of the yeast Saccharomyces cerevisiae in the wine-making context. Two solution
methods are compared: a metabolic flux analysis (MFA) using convex analysis and providing narrow
intervals of variation for the fluxes, and a flux balance analysis (FBA) based on an objective function.
The constraints allowing the solution of the underdetermined set of algebraic equations are typically
originating from measurements of uptake and secretion rates of external metabolites, and/or the use of an
objective function, and/or metabolic constraints. It is shown here that converting reactions schemes in
Cmol unit combined with data reconciliation provides a convenient formulation of closed carbon balance.
In this form, the constraint formulation is natural and facilitates the understanding of the carbon
distribution within the yeast metabolism.
Fichier principal
Closed carbon balance in calculation of metabolic fluxes_1.pdf (310.03 Ko)
Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...