Enzymatic analysis normalization in vine soils: determination of quality and biological productivity

Alejandro Alonso (1) , Nathalie Cheviron (2, 3) , Eva Navascués (4) , Antonio Santos (1) , Domingo Marquina (1)

1 Departamento de Microbiología III [Madrid]
2 ECOSYS - Ecologie fonctionnelle et écotoxicologie des agroécosystèmes
3 Université Paris-Saclay
4 Pago de Carraovejas S.L.

Dehydrogenase activity enzymatic determination and the measurement of Substrate Induced Respiration (SIR) are methods that allow microbial processes analysis produced in the soil. Enzymes such as phosphatases and β-galactosidase, can be considered as specific parameters, integrated in the cycle of phosphorus and carbon. Measurement of all these activities allows to know soil activity metabolic and behavior of all. Our research team has collaborated on the standardization project: ISO/CD20130 "Measurement of enzyme activity patterns in soil samples using colorimetric substrates in micro-well plates”, led by Nathalie Cheviron, technical director of BiochemENV platform (INRA, Versailles). Since 2014, we have been managing an integral microbiota study associated with singular organic vineyards (CDTI IDI 20140448) in Ribera de Duero. Our objective is to determine fertility indexes based on the microbial groups present and their most representative metabolic activities. Due to the robustness, repeatability and specificity of the ISO/CD20130 technique, we have implemented the measurement standard of soil enzymatic activities in our laboratory. Being interesting for technical applications in viticulture to guarantee a sustainable end product with unique characteristics

Domaines: Sciences du Vivant [q-bio]

Alejandro Alonso, Nathalie Cheviron, Eva Navascués, Antonio Santos, Domingo Marquina. Enzymatic analysis normalization in vine soils: determination of quality and biological productivity. 40th World Congress of Vine and Wine, May 2018, Sofia, Bulgaria. , 2018. ⟨hal-01812808⟩