Direct and Rapid Profiling of Biophenols in Olive Pomace by UHPLC-DAD-MS

Aurélia Malapert (1) , Emmanuelle Reboul (2) , Michele Loonis (1) , Olivier Dangles (1) , Valérie Tomao (1)

1 SQPOV - Sécurité et Qualité des Produits d'Origine Végétale
2 NORT - Nutrition, obésité et risque thrombotique

Olive mill by-products are effluents generated during olive oil production process. The two-phase centrifugation system produces a semi-solid olive pomace called “alperujo.” This by-product is a combination of liquid and solid wastes derived from the three-phase manufacturing process. A direct and fast analytical method by ultra-high-performance liquid chromatography-DAD coupled with ESI/MS-MS has been developed for the profiling of phenolic compounds. Thirty-five metabolites belonging to phenyl alcohols, secoiridoids, flavonoids, and iridoids were identified as the main constituents of alperujo in 12 min, including p-coumaroyl aldarate and a verbascoside derivative found for the first time in alperujo and a new ligstroside derivative. Six quantitatively significant components were determined at concentrations ranging from 17.7 mg/L for p-coumaric acid to 370.7 mg/L for hydroxytyrosol. Our data confirm that alperujo is an interesting source of phenolic compounds that could be extracted for use as nutraceuticals.

Mots clés: phenolic compound; centrifuging; Fast analysis; UHPLC/MS; Alperujo; Two-phase olive pomace; Olive phenolic compounds; Biophenols; coumaric acids; nutraceutical

Mots clés: profilage génétique; production d'olives; oléiculture; centrifugation; composé phénolique; hydroxytyrosol; acide coumarique; grignon; huile d'olive; nutraceutique

Domaines: Alimentation et Nutrition

Aurélia Malapert, Emmanuelle Reboul, Michele Loonis, Olivier Dangles, Valérie Tomao. Direct and Rapid Profiling of Biophenols in Olive Pomace by UHPLC-DAD-MS. Food Analytical Methods, 2017, 11 (4), pp.1-10. ⟨10.1007/s12161-017-1064-2⟩. ⟨hal-01709338⟩