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H. Y. Ho, R. Rohatgi, A. M. Lebensohn, M. Le, J. Li et al., Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex 203-216 FIGURE LEGENDS Figure 1. ?1 integrins regulate osteoblast cell proliferation in vivo and in vitro. A. Histo-morphometric analysis of osteoblast number on wild-type (?1 f/f ) and Osx-Cre; ?1 f/f (?1 Ost-KO ) 30-days-old mice tibias. Graphs show the mean ± SD from five independent experiments B. Quantification of apoptotic (TUNEL-positive) and proliferating (BrdU-positive) cells in periosteum and trabecular bone in wild-type and mutant 30-days-old mice tibias (p; periosteum; t; trabecular bone). n=50; statistical significance of differences assessed by a two-tailed unpaired Student's t-test, 3 independent experiments. C. Representative TUNEL staining D. BrdU staining on trabecular bone sections from wild-type and mutant mouse tibias (hc, hypertrophic cartilage; tb, trabecular bone; bm, bone marrow) Scale bar represent 40µm. E. Images of BrdU staining of trabecular bone sections. F. BrdU based quantification of the proliferation rate of ?1f/f, ?1-/-primary mouse embryonic fibroblasts. (statistical significance of differences assessed by a two-tailed unpaired Student's t-test, 3 independent experiments) Cell adhesion control of YAP activation 17 G. In vitro proliferation rate of wild-type (?1 f/f ) and ?1 integrin deficient (?1 -/-) osteoblasts. n=50; statistical significance of differences assessed by a two-tailed unpaired Student's t-test, 3 independent experiments. H. BrdU based quantification of the proliferation rate of ?1 f Statistical significance of differences assessed by a two-tailed unpaired Student's t-test, osteoblasts, or ?1 -/osteoblasts expressing human ?1 integrin (rescue), constitutively active MEK (MEKQ56P), or nuclear active ERK fusion mutant (MEK/ERKLA), 2004.

G. Ras and . Mefs, Nuclei were stained with DAPI F. Western blot analysis of YAP phosphorylation. YAP pS127 and total YAP in ?1 f/f and ?1 -/osteoblasts after cell fractionation of the nuclear fraction (N) and cytoplasmic/membrane fraction (CM). Lamin B and tubulin were used as nuclear and cytoplasmic markers

F. Rt, CyclinD2 (CCND2), p19Arf and p21CIP (CDKN1A) mRNAs in ?1 -/-and ?1 f/f osteoblasts expressing Flag-YAP 5SA normalized to ?1 f/f osteoblasts, CCND1)