Skip to Main content Skip to Navigation
Journal articles

Prognostic value of the Hippo pathway transcriptional coactivators ă YAP/TAZ and beta 1-integrin in conventional osteosarcoma

Abstract : Introduction: Currently, very few studies are available concerning the ă mammalian Hippo pathway in bone sarcomas. YAP/TAZ transcription ă co-activators are key downstream effectors of this pathway and may also ă have oncogenic properties. Additionally, recent in-vitro experiments ă showed that expression of beta 1-integrin promoted metastasis in ă osteosarcomas. This study investigated the expression of YAP/TAZ and ă beta 1-integrin in human osteosarcomas. Materials and methods: We performed automated immunohistochemistry on ă tissue-microarrays (TMA) in which 69 conventional osteosarcomas biopsies ă performed prior to chemotherapy were embedded. Cellular localization and ă semi-quantitative analysis of each immunostain was performed using ă Immunoreactive Score (IRS) and correlated to clinico-pathological data. Results: Cytoplasmic expression of beta 1-integrin was noted in 54/59 ă osteosarcomas (92%), with 33/59 cases (56%) displaying membranous ă staining. YAP/TAZ was expressed in 27/45 osteosarcomas (60%), with 14 ă cases (31%) showing cytoplasmic expression while 13 other cases (28%) ă displayed nuclear expression. No link was found between YAP/TAZ or beta ă 1-integrin expression and response to chemotherapy. In univariate ă analysis, YAP/TAZ immunoreactive score was pejoratively correlated with ă overall survival (p = 0.01). Expression of beta 1-integrin on cell ă membrane was also pejorative for OS (p = 0.045). In multivariate ă analysis, YAP/TAZ nuclear expression was an independent prognostic ă factor for PFS (p = 0.035). Conclusion: this study indicates that beta 1-integrin and YAP/TAZ ă proteins are linked to prognosis and therefore could be therapeutic ă targets in conventional osteosarcomas.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-01482390
Contributor : Pascal Auquier Connect in order to contact the contributor
Submitted on : Wednesday, May 5, 2021 - 11:38:28 AM
Last modification on : Tuesday, October 19, 2021 - 10:59:45 PM
Long-term archiving on: : Friday, August 6, 2021 - 6:30:25 PM

File

11876-179003-4-PB.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Corinne Bouvier, Nicolas Macagno, Quy Nguyen, Anderson Loundou, Carine Jiguet-Jiglaire, et al.. Prognostic value of the Hippo pathway transcriptional coactivators ă YAP/TAZ and beta 1-integrin in conventional osteosarcoma. Oncotarget, Impact journals, 2016, 7 (40), pp.64702-64710. ⟨10.18632/oncotarget.11876⟩. ⟨hal-01482390⟩

Share

Metrics

Record views

327

Files downloads

57