Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge
Résumé
Negative Energy Balance (NEB) is considered to increase susceptibility to mastitis. The objective of this
study was to improve our understanding of the underlying mechanisms by comparing transcriptomic
profiles following NEB and a concomitant mammary inflammation. Accordingly, we performed RNAseq
analysis of blood cells in energy-restricted ewes and control-diet ewes at four different time points
before and after intra mammary challenge with phlogogenic ligands. Blood leucocytes responded
to NEB by shutting down lipid-generating processes, including cholesterol and fatty acid synthesis,
probably under transcriptional control of SREBF 1. Furthermore, fatty acid oxidation was activated and
glucose oxidation and transport inhibited in response to energy restriction. Among the differentially
expressed genes (DEGs) in response to energy restriction, 64 genes were also differential in response
to the inflammatory challenge. Opposite response included the activation of cholesterol and fatty acid
synthesis during the inflammatory challenge. Moreover, activation of glucose oxidation and transport
coupled with the increase of plasma glucose concentration in response to the inflammatory stimuli
suggested a preferential utilization of glucose as the energy source during this stress. Leucocyte
metabolism therefore undergoes strong metabolic changes during an inflammatory challenge, which
could be in competition with those induced by energy restriction.
Domaines
Sciences du Vivant [q-bio]
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