Methods for Proteome Analysis of Campylobacter jejuni Using 2-D Electrophoresis

Ramila Christiane Rodrigues (1, 2) , Nabila Haddad (1, 2) , Odile Tresse (1, 2)

1 SECALIM - SECurité des ALIments et Microbiologie
2 UNAM - PRES Université Nantes Angers Le Mans

This chapter describes protocols used for two-dimensional electrophoretic analysis of the proteome or subproteome of Campylobacter jejuni, a major human food-borne pathogen. The following protocols, adapted to Campylobacter strains, include all the steps from cultivation to gel-support protein separation.

Mots clés: 2-D electrophoresis; Campylobacter; Protein extraction; Protein purification; Isoelectric point

Domaines: Sciences du Vivant [q-bio]

Ramila Christiane Rodrigues, Nabila Haddad, Odile Tresse. Methods for Proteome Analysis of Campylobacter jejuni Using 2-D Electrophoresis. Campylobacter jejuni, 1512, HUMANA PRESS INC, pp.12, 2017, Methods in Molecular Biology, 978-1-4939-6536-6; 978-1-4939-6534-2. ⟨10.1007/978-1-4939-6536-6_19⟩. ⟨hal-01603243⟩