Skip to Main content Skip to Navigation
Journal articles

Impact of Orexin-A treatment on food intake, energy metabolism and body weight in mice

Abstract : Orexin-A and -B are hypothalamic neuropeptides of 33 and 28-amino acids, which regulate many homeostatic systems including sleep/wakefulness states, energy balance, energy homeostasis, reward seeking and drug addiction. Orexin-A treatment was also shown to reduce tumor development in xenografted nude mice and is thus a potential treatment for carcinogenesis. The aim of this work was to explore in healthy mice the consequences on energy expenditure components of an orexin-A treatment at a dose previously shown to be efficient to reduce tumor development. Physiological approaches were used to evaluate the effect of orexin-A on food intake pattern, energy metabolism body weight and body adiposity. Modulation of the expression of brain neuropeptides and receptors including NPY, POMC, AgRP, cocaine-and amphetamine related transcript (CART), corticotropin-releasing hormone (CRH) and prepro-orexin (HCRT), and Y-2 and Y-5 neuropeptide Y, MC4 (melanocortin), OX1 and OX2 orexin receptors (Y2R, Y5R, MC4R, OX1R and OX2R, respectively) was also explored. Our results show that orexin-A treatment does not significantly affect the components of energy expenditure, and glucose metabolism but reduces intraperitoneal fat deposit, adiposity and the expression of several brain neuropeptide receptors suggesting that peripheral orexin-A was able to reach the central nervous system. These findings establish that orexin-A treatment which is known for its activity as an inducer of tumor cell death, do have minor parallel consequence on energy homeostasis control.
Document type :
Journal articles
Complete list of metadata

Cited literature [47 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01602768
Contributor : Archive Ouverte Prodinra <>
Submitted on : Tuesday, May 26, 2020 - 10:28:27 AM
Last modification on : Wednesday, January 27, 2021 - 3:42:07 PM

File

2017_Blais_Plos One_1.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Anne Blais, Gaëtan Drouin, Catherine Chaumontet, Thierry Voisin, Anne Couvelard, et al.. Impact of Orexin-A treatment on food intake, energy metabolism and body weight in mice. PLoS ONE, Public Library of Science, 2017, 12 (1), pp.1-14. ⟨10.1371/journal.pone.0169908⟩. ⟨hal-01602768⟩

Share

Metrics

Record views

420

Files downloads

184