Molecular characterisation of epizootic haemorrhagic disease virus associated with a tunisian outbreak among cattle in 2006

Sameh Ben Dhaou (1, 2, 3, 4, 5) , Corinne Sailleau (3) , Besma Babay (2) , Cyril Viarouge (3) , Soufien Sghaier (2) , Stéphan Zientara (3) , Salah Hammami (6, 2) , Emmanuel Bréard (3)

1 Faculté des Sciences de Bizerte [Université de Carthage]
2 IRVT - Institut de Recherche Vétérinaire de Tunisie
3 VIRO - Virologie UMR1161
4 ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
5 UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12
6 École Nationale de Médecine Vétérinaire de Sidi Thabet

In 2006, epizootic haemorrhagic disease (EHD) outbreaks were recorded in the Maghreb (Tunisia, Morocco and Algeria) among cattle, resulting in severe repercussions on herds (oedema of the head, necrotic lesions of the oral mucosa, hyperthermia of the teats, accompanied by anorexia and respiratory distress) and economic losses. The present study gives new information on the molecular characterisation of the EHD virus (EHDV) that had circulated in Tunisia. Genome segments 2, 3, 6, 7 and 10 of EHDV, corresponding to the VP2, VP3, VP5, VP7 and NS3/NS3A proteins, respectively, were amplified from the blood of one animal by RT-PCR and sequenced. Nucleotide sequence comparisons of these five segments with sequences available in the GenBank demonstrated that an EHDV serotype 6 (EHDV-6) had been present in Tunisia in 2006. The possible origin of this strain is discussed.

Mots clés: Epizootic haemorrhagic disease virus; serotype 6; RT-PCR; sequencing; phylogenetic tree

Domaines: Sciences du Vivant [q-bio]

Sameh Ben Dhaou, Corinne Sailleau, Besma Babay, Cyril Viarouge, Soufien Sghaier, et al.. Molecular characterisation of epizootic haemorrhagic disease virus associated with a tunisian outbreak among cattle in 2006. Acta Veterinaria Hungarica, 2016, 64 (2), pp.250-262. ⟨10.1556/004.2016.025⟩. ⟨hal-01602395⟩