Material influence on biocontamination level and adhering cell physiology

Audrey Allion (1) , Jean-Marie Herry (2, 3) , Marie Noelle Bellon-Fontaine (2, 3)

1 APERAM
2 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
3 Université Paris-Saclay

In most environments, association with a surface in a structure known as a biofilm is the prevailing microbial lifestyle. Several factors may influence the biofilm formation e.g. nutrients, temperature, flow velocity, initial microflora and the nature of materials. Considering the biocontamination mechanism described in four steps, the initial adhesion is a key element in the biocontamination phenomenon and the substratum is of major concern in controlling bacterial adhesion. Stainless steel is well used in numerous markets because of its high cleanability and corrosion resistance properties. However, other materials are put forward by focusing on properties which differentiate them from those of stainless steel. Thereby, to select the material best suited to the problem, there should have data on their aptitude for biocontamination as well as adhesion impact on cell physiology. For all materials, the ratio of dead adhering cells is lower than 55%. The results obtained show that cell injury is not higher on material known to be bactericidal than on other ones.

Dans la plupart des environnements, les microorganismes vivent pr ́ ef ́ erentiellement au sein de biofilms. De nombreux facteurs influencent leur formation i.e. les nutriments, la temp ́ erature, le r ́ egime du fluide environnent, la microflore et les mat ́ eriaux. Dans le m ́ ecanisme de biocontamination, d ́ ecrit en quatre ́ etapes successives, l’adh ́ esion initiale est un ́ el ́ ement cl ́ e de la bioadh ́ esion et les mat ́ eriaux un ́ el ́ ement majeur pour son contr ˆ ole. L’acier inoxydable est tr ` es utilis ́ e dans de nombreux secteurs d’activit ́ e pour sa bonne nettoyabilit ́ e et son excellente r ́ esistance ` a la corrosion. Pour se diff ́ erencier, certains mat ́ eriaux mettent en avant d’autres propri ́ et ́ es.Ainsi,las ́ election du mat ́ eriau le mieux adapt ́ e ` a un probl ` eme donn ́ en ́ ecessite de connaitre son aptitude ` a la biocontamination ainsi que son impact sur la physiologie des microorganismes. Pour tous les mat ́ eriaux test ́ es, la mortalit ́ e des bact ́ eries adh ́ erentes est inf ́ erieure ` a55%.Lesr ́ esultats obtenus ont montr ́ e qu’un mat ́ eriau dit antimicrobien n’induit pas plus de cellules endommag ́ ees comparativement aux autres mat ́ eriaux.

Domaines: Sciences du Vivant [q-bio]

Audrey Allion, Jean-Marie Herry, Marie Noelle Bellon-Fontaine. Material influence on biocontamination level and adhering cell physiology. Journées annuelles de la Société Française de Matériaux et de Métallurgie (SF2M), Oct 2013, Villeneuve d'Ascq, France. EDP Sciences, MATEC Web of Conferences, 7, 2013, MATEC Web of Conferences. ⟨10.1051/matecconf/20130704004⟩. ⟨hal-01601858⟩