Bacteria isolated from lung modulate asthma susceptibility in mice

Aude Remot (1, 2) , Delphyne Descamps (3, 1) , Marie-Louise Noordine (4, 1) , Abdelhak Boukadiri (5) , Elliot Mathieu (4, 1) , Véronique Robert (4, 1) , Sabine Riffault (3, 1) , Bart Lambrecht (6, 7) , Philippe Langella (4, 1) , Hamida Hammad (6, 7) , Muriel Thomas (4, 1)

1 Université Paris-Saclay
2 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
3 VIM (UR 0892) - Unité de recherche Virologie et Immunologie Moléculaires
4 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
5 GABI - Génétique Animale et Biologie Intégrative
6 VIB Inflammation Research Center
7 Department of Respiratory Medicine

Asthma is a chronic, non-curable, multifactorial disease with increasing incidence in industrial countries. This study evaluates the direct contribution of lung microbial components in allergic asthma in mice. Germ-Free and Specific-Pathogen-Free mice display similar susceptibilities to House Dust Mice-induced allergic asthma, indicating that the absence of bacteria confers no protection or increased risk to aeroallergens. In early life, allergic asthma changes the pattern of lung microbiota, and lung bacteria reciprocally modulate aeroallergen responsiveness. Primo-colonizing cultivable strains were screened for their immunoregulatory properties following their isolation from neonatal lungs. Intranasal inoculation of lung bacteria influenced the outcome of allergic asthma development: the strain CNCM I 4970 exacerbated some asthma features whereas the pro-Th1 strain CNCM I 4969 had protective effects. Thus, we confirm that appropriate bacterial lung stimuli during early life are critical for susceptibility to allergic asthma in young adults

Mots clés: lung; mice; bronchial asthma

Mots clés: poumon; souris; microbiote; asthme

Domaines: Sciences du Vivant [q-bio]

Aude Remot, Delphyne Descamps, Marie-Louise Noordine, Abdelhak Boukadiri, Elliot Mathieu, et al.. Bacteria isolated from lung modulate asthma susceptibility in mice. The International Society of Microbiologial Ecology Journal, 2017, pp.1061-1074. ⟨10.1038/ismej.2016.181⟩. ⟨hal-01595509⟩