Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains

Wanderson Silva (1, 2) , Edson L. Folador (3) , Siomar C. Soares (2, 4) , Gustavo H. M. F. Souza (5) , Agenor V. Santos (6) , Cassiana S. Sousa (2) , Henrique Figueiredo (7) , Anderson Miyoshi (2) , Yves Le Loir (1) , Artur Silva (8) , Vasco Azevedo (2)

1 STLO - Science et Technologie du Lait et de l'Oeuf
2 ICB - Instituto de Ciencias Biologicas [Minas Gerais]
3 Centro de Biotecnologia
4 Departmento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Biológicas e Naturais
5 Waters Corporation, Waters Technologies Brazil, MS Applications Laboratory, Alphaville
6 Instituto de Ciências Biológicas
7 Departamento de Medicina Veterinária Preventiva, Escola de Veterinária
8 Instituto de Ciências Biológicas

Background Corynebacterium pseudotuberculosis is a pathogen classified into two biovars: C. pseudotuberculosis biovar ovis, the etiologic agent of caseous lymphadenitis and C. pseudotuberculosis biovar equi, which causes ulcerative lymphangitis. The available whole genome sequences of different C. pseudotuberculosis strains have enabled identify difference of genes related both virulence and physiology of each biovar. To evaluate be this difference could reflect at proteomic level and to better understand the shared factors and the exclusive ones of biovar ovis and biovar equi strains, we applied the label-free quantitative proteomic to characterize the proteome of the strains: 1002_ovis and 258_equi, isolated from goat (Brazil) and equine (Belgium), respectively. Results From this analysis, we characterized a total of 1230 proteins in 1002_ovis and 1220 in 258_equi with high confidence. Moreover, the core-proteome between 1002_ovis and 258_equi obtained here is composed of 1122 proteins involved in different cellular processes, which could be necessary for the free living of C. pseudotuberculosis. In addition, 120 proteins from this core-proteome presented change in abundant with statistically significant differences. Considering the exclusive proteome, we detected strain-specific proteins to each strain. When correlated, the exclusive proteome of each strain and proteome with change in abundant, the proteomic differences, between the 1002_ovis and 258_equi, this related to proteins involved in cellular metabolism, information storage and processing, cellular processes and signaling. Conclusions This study reports the first comparative proteomic study of the biovars ovis and equi of C. pseudotuberculosis. The results generated in this study provide information about factors which can contribute to understanding both the physiology and the virulence of this pathogen.

Mots clés: protéomique; Caseous lymphadenitis; Ulcerative lymphangitis; animal health

Mots clés: corynebacterium pseudotuberculosis; souche bactérienne; approche protéomique; caractérisation souche bactérienne; séquence génomique; santé animale

Domaines: Alimentation et Nutrition; Ingénierie des aliments; Microbiologie et Parasitologie

Wanderson Silva, Edson L. Folador, Siomar C. Soares, Gustavo H. M. F. Souza, Agenor V. Santos, et al.. Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains. BMC Genomics, 2017, 18 (1), open acess. ⟨10.1186/s12864-017-3835-y⟩. ⟨hal-01593194⟩