Correlative Imaging of Structural and Elemental Composition of Bacterial Biofilms

Y. Yang (1) , R. Heine (1) , F. Xu (1) , H. Suhonen (2) , L. Helfen (1, 2) , A. Rosenhahn , T. Gorniak , S. Kirchen , T. Schwartz , T. Baumbach (1)

1 KIT - Karlsruhe Institute of Technology
2 ESRF - European Synchrotron Radiation Facility

Synchrotron-based phase contrast tomography (holotomography) and scanning hard X-ray fluorescence microscopy (SXFM) are combined to characterize the three-dimensional (3D) structural and corresponding elemental distribution of bacterial biofilms of Pseudomonas aeruginosa. Samples were fixed without contrast agents or microtomal sectioning. Within an intact microbial community single bacteria are clearly resolved, and their morphology can be directly visualized together with the elemental content. Such 3D set of complementary information at cellular level is essential for gaining a deeper understanding of biofilm evolution aiming to develop potential strategies on biofilm growth control and prevention

Domaines: Physique [physics]

Y. Yang, R. Heine, F. Xu, H. Suhonen, L. Helfen, et al.. Correlative Imaging of Structural and Elemental Composition of Bacterial Biofilms. 11th International Conference on X-ray Microscopy (XRM), Aug 2012, Shanghai, China. 4 p., ⟨10.1088/1742-6596/463/1/012053⟩. ⟨hal-01572854⟩