3-Hydroxypropionaldehyde (3-HPA) quantification by HPLC using a synthetic acrolein-free 3-hydroxypropionaldehyde system as analytical standard

Grégoire Burgé (1, 2) , Amandine Flourat (3, 2) , Brigitte Pollet (1) , Henry-Eric Spinnler (1) , Florent Allais (1, 2)

1 GMPA - Génie et Microbiologie des Procédés Alimentaires
2 ABI - Agro-Biotechnologies Industrielles
3 IJPB - Institut Jean-Pierre Bourgin

HPLC-based quantification of 3-HPA using a synthetic acrolein-free 3-HPA standard obtained from commercially available 1,2,4-butanetriol through a straightforward and easy synthetic process has advantages over previous colorimetric methods of easier and safer implementation, and greater specificity. This HPLC method is very simple to implement in a lab, does not need any extra handling of the sample to be analyzed, and is suitable even in the presence of other aldehydes and 3-HPA derivatives, provided that the latter do not have similar retention times.

Mots clés: lactobacillus-reuteri; glycerol bioconversion; hydroxypropionaldehyde; pathogens; stability; growth; acid

Domaines: Sciences du Vivant [q-bio]

Grégoire Burgé, Amandine Flourat, Brigitte Pollet, Henry-Eric Spinnler, Florent Allais. 3-Hydroxypropionaldehyde (3-HPA) quantification by HPLC using a synthetic acrolein-free 3-hydroxypropionaldehyde system as analytical standard. RSC Advances, 2015, 5 (112), pp.92619 - 92627. ⟨10.1039/c5ra18274c⟩. ⟨hal-01535299⟩