Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus.

Fethi Ben Abdallah (1, 2) , Kamel Chaieb (1) , Tarek Zmantar (1) , Héla Kallel (2) , Amina Bakhrouf (1)

1 LATVPEP - Laboratoire d’analyse, de traitement et de valorisation des polluants de l’environnement et des produits [Monastir]
2 Institut Pasteur de Tunis

In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrio parahaemolyticus strains, food-borne pathogens, using a Congo red agar plate assay. Furthermore, we studied their ability to adhere to abiotic surfaces and Vero cells line. Our results showed that only V. alginolyticus ATCC 17749 was a slime-producer developing almost black colonies on Congo red agar plate. Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V. parahaemolyticus. Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells (OD(570) = 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after 60 min all the studied strains become adherent. The percentage of adherence ranged from 1.23% to 4.66%.

Mots clés: adherence; slime production; abiotic surface; Vero cells; Vibrio

Domaines: Sciences du Vivant [q-bio]

Fethi Ben Abdallah, Kamel Chaieb, Tarek Zmantar, Héla Kallel, Amina Bakhrouf. Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus.. Brazilian Journal of Microbiology, 2009, 40 (2), pp.394-398. ⟨10.1590/S1517-838220090002000033⟩. ⟨hal-01358556⟩