ANTIBIOTIC SUSCEPTIBILITY PATTERN OF SALMONELLA SPECIES AND OTHER MICROORGANISMS ISOLATED FROM SACHETS WATER SOLD AND CONSUMED IN NIGER DELTA REGION OF NIGERIA

Olorode Oluwayemisi Agnes (1) , Emmanuel Bamigbola (2) , Peace Iroele Chinomnso (1)

1 Department of Pharmaceutical Microbiology and Biotechnology
2 Department of Pharmaceutics

Sachet water in Nigeria is popularly called 'Pure Water'. The demand for safe drinking water in Nigeria cannot be overemphasized considering the inability of the government to provide adequate pipe borne water to the populace. However, safe drinking water is very scarce, the ever increasing demand of readily available water has led to the concept of pure water. Is this sachet water really safe for drinking? This study was carried out to examine the microbiological quality of sachet drinking water sold in Bayelsa and Rivers States, Niger Delta regions of Nigeria Different brands of sachets water sold and consumed in Rivers and Bayelsa states (Niger Delta regions of Nigeria) were studied for the presence of Salmonella species and other enteric microorganisms between January and October 2012. Thirty five (35) sachets water from seven (7) different brands were serially diluted and cultured on selective media Mac Conkey, Salmonella Shigella and Eosin Methylene Blue agar and Nutrient agar for stock culture, while Muller Hinton agar was used for sensitivity test. Pure isolates suspension was standardized with 0.5 Mc Farland turbidity standard and were subjected to antibiotics susceptibility test using Agar Diffusion method and Biomark antibiotic disc. Bottle water was also tested as control. Various bacteria were isolated from all the test sachets water except the bottle water. The microbial counts ranged from 1.9 x 10 2 Cfu/ml to 6.3 x 10 5 Cfu/ml. Bacteria isolates include Salmonella sp. 16 strains, Streptococcus sp. 12 strains, Klebsiella sp. 6 strains, Escherichia coli 2 strains, Bacillus sp. 7 strains, Enterococcus sp. 4, and Enterobacter sp. 2. All the isolates were highly susceptible to Fluoroquinolones class of antibiotics, Ofloxacin (98.1%) Pefloxacin (99.2%), Levofloxacin (95.5%), Ciprofloxacin (99.5%), Streptomycin (94.4%) and Aminoglycosides Gentamycin (94.4%); moderately susceptible to Penicillin class, Amoxicillin (55.2%), Ampiclox (54.2%), Rifampicin (65.7%) and Macrolides, Erytromycin (64.1%) and highly resistant to Nalidixic Acid, Ampicillin and Ceporex. Statistical analysis showed no significant difference in the efficacy of the antibiotics susceptibility at (P<0.05). These findings showed that the quality of test sachets water does not meet the World Health Organization Standard and should be discouraged from drinking. The drug pattern in this study may serve as a guide in patient care.

Mots clés: Salmonella species; Bacteria; Sachet water; Antimicrobial susceptibility

Domaines: Bactériologie

Olorode Oluwayemisi Agnes, Emmanuel Bamigbola, Peace Iroele Chinomnso. ANTIBIOTIC SUSCEPTIBILITY PATTERN OF SALMONELLA SPECIES AND OTHER MICROORGANISMS ISOLATED FROM SACHETS WATER SOLD AND CONSUMED IN NIGER DELTA REGION OF NIGERIA. Continental J. Pharmaceutical Sciences , 2014, 8 (1), pp.1-6. ⟨hal-01326643⟩