Exploring the Transcriptome of Mycorrhizal Interactions
Résumé
The transcriptome is defined as the sum of all transcripts in a given cell, tissue or organ and can be assessed by different approaches. The most recent ones, microarrays and RNA sequencing, are compared in this chapter with a focus on plant microbe interactions. The advantages and the specific problems to monitor both partners at the same time by sequencing are discussed. We show that microdissection in combination with transcriptomics is a powerful tool to study interactions. Further, we present an overview of bioinformatic approaches and available algorithms to process RNA-Seq data. Finally, we summarize the transcriptome analyses from mycorrhizal fungi in the genome era, in particular Laccaria bicolor, Tuber melanosporum, Rhizophagus irregularis and Paxillus involutus.