First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)

Virginie Molinier (1) , Claude Murat-Furminieux (2) , Emmanuelle Morin (2) , Armelle Gollotte (3) , Daniel Wipf (1) , Francis Martin (2)

1 Agroécologie [Dijon]
2 IAM - Interactions Arbres-Microorganismes
3 Inoplant

Tuber aestivum, the most common truffle in Europe, tends to take an important place in the truffle economic market. For the first time, microsatellites primers were developed to investigate the polymorphism within this species. Using direct shotgun pyrosequencing, 15 polymorphic microsatellites were identified out of the 7,784 perfect microsatellites present in the 534,620 reads obtained. Tested on 75 samples, these microsatellites were highly polymorphic. Alleles varied from 4 to 15 and the expected heterozygosity from 0.266 to 0.620. A multi-locus analysis allowed the identification of 63 genotypes over the 75 samples analyzed. Direct shotgun pyrosequencing is a fast and relatively low cost technique allowing identification of microsatellites in non-model species. The microsatellites developed here will allow performing a genetic population study to infer the evolutionary history of this species.

Mots clés: Tuber aestivum; Tuber uncinatum; microsatellites markers; pyrosequencing

Domaines: Sciences du Vivant [q-bio]

Virginie Molinier, Claude Murat-Furminieux, Emmanuelle Morin, Armelle Gollotte, Daniel Wipf, et al.. First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae). 3. Annual Joint Meeting on Plant Microbe Interactions, Sep 2014, Dijon, France. ⟨hal-01268760⟩