Functionality of Sortase A in Lactococcus lactis

Yakhya Dieye (1) , Virginie Oxaran (1) , Florence Ledue (1) , Walid Alkhalaf (1) , Girbe Buist (2) , Vincent Juillard (1) , Chang Won Lee (3) , Jean-Christophe Piard (1)

1 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
2 Dept Mol Genet, Groningen Biomol Sci & Biotechnol Inst
3 Div Life Sci, Lab Proteome Anal & Mol Physiol

Lactococcus lactis IL1403 harbors a putative sortase A (SrtA) and 11 putative sortase substrates that carry the canonical LPXTG signature of such substrates. We report here on the functionality of SrtA to anchor five LPXTG substrates to the cell wall, thus suggesting that SrtA is the housekeeping sortase in L. lactis IL1403.

Mots clés: GRAM-POSITIVE BACTERIA; CELL-WALL; STREPTOCOCCUS-PYOGENES; SURFACE-PROTEINS; ACBACTERIA; BACILLUS-SUBTILIS; BINDING DOMAIN; SUBSP LACTIS; E7 ANTIGEN; GENE

Domaines: Sciences du Vivant [q-bio]

Yakhya Dieye, Virginie Oxaran, Florence Ledue, Walid Alkhalaf, Girbe Buist, et al.. Functionality of Sortase A in Lactococcus lactis. Applied and Environmental Microbiology, 2010, 76 (21), pp.7332 - 7337. ⟨10.1128/AEM.00928-10⟩. ⟨hal-01204271⟩