Microsatellites cross-species amplification across some african cichlids

Etienne Bezault (1, 2, 3) , Xavier X Rognon (2) , Karim Gharbi (2, 4) , Jean-François Baroiller (1) , Bernard Chevassus-Au-Louis (2)

1 UMR INTREPID - Intensification raisonnée et écologique pour une pisciculture durable
2 GABI - Génétique Animale et Biologie Intégrative
3 Department of Biology
4 Institute of Evolutionary Biology, School of Biological Sciences

The transfer of the genomic resources developed in the Nile tilapia, Oreochromis niloticus, to other Tilapiines sensu lato andAfrican cichlid would provide new possibilities to study this amazing group from genetics, ecology, evolution, aquaculture, andconservation point of view.We tested the cross-species amplification of 32 O. niloticus microsatellite markers in a panel of 15 speciesfrom 5 different African cichlid tribes: Oreochromines (Oreochromis, Sarotherodon), Boreotilapiines (Tilapia), Chromidotilapines,Hemichromines, and Haplochromines. Amplification was successfully observed for 29 markers (91%), with a frequency ofpolymorphic (P95) loci per species around 70%. The mean number of alleles per locus and species was 3.2 but varied from 3.7within Oreochromis species to 1.6 within the nontilapia species. The high level of cross-species amplification and polymorphism ofthe microsatellite markers tested in this study provides powerful tools for a wide range of molecular genetic studies within tilapiaspecies as well as for other African cichlids.

Mots clés: cichlid; amplification; satellite

Domaines: Sciences du Vivant [q-bio]

Etienne Bezault, Xavier X Rognon, Karim Gharbi, Jean-François Baroiller, Bernard Chevassus-Au-Louis. Microsatellites cross-species amplification across some african cichlids. International Journal of Evolutionary Biology, 2012, 2012, Non paginé. ⟨10.1155/2012/870935⟩. ⟨hal-01191065⟩