Design and in vitro studies of a needle-type glucose sensor for subcutaneous monitoring

Dilbir S. Bindra (1) , Yanan Zhang (1) , George S. Wilson (1) , Robert Sternberg (2) , Thévenot Daniel R. (3, 2) , Dinah Moatti (4) , Gérard Reach (4)

1 Department of Chemistry [Kansas]
2 LABAM - Laboratoire de Bioélectrochimie et Analyse du Milieu
3 CEREVE - Centre d'Enseignement et de Recherche Eau Ville Environnement
4 Service de Diabétologiè, Hôtel-Dieu de Paris

A new miniaturized glucose oxidase based needle-type glu¬ cose mlcrosensor has been developed for subcutaneous glu¬ cose monitoring. The sensor Is equivalent In shape and size to a 26-gauge needle (0.45-mm o.d.) and can be Implanted with ease without any Incision. The novel configuration greatly facilitates the deposition of enzyme and polymer films so that sensors with characteristics suitable for In vivo use (upper limit of linear range > 15 mM, response time < 5 mln, and sensitivity yielding a 5:1 signal-to-background ratio at normal basal glucose levels) can be prepared In high yield (>60%). The sensor response is largely Independent of ox¬ ygen tension In the normal physiological range. It also ex¬ hibits good selectivity against common interferences except for the exogenous drug acetaminophen.

Domaines: Chimie; Chimie analytique; Biochimie [q-bio.BM]; Chimie théorique et/ou physique; Biotechnologies

Dilbir S. Bindra, Yanan Zhang, George S. Wilson, Robert Sternberg, Thévenot Daniel R., et al.. Design and in vitro studies of a needle-type glucose sensor for subcutaneous monitoring. Analytical and Bioanalytical Chemistry, 1991, 63, pp.1692-1696. ⟨10.1021/ac00017a008⟩. ⟨hal-01179862⟩