Relationship between nanoscale mineral properties and calcein labeling in mineralizing bone surfaces

Marta Aido (1) , Michael Kerschnitzki (1, 2) , Rebecca Hoerth (1, 2) , Manfred Burghammer (3, 4) , Cédric Montero (3) , Sara Checa (1) , Peter Fratzl (1, 2) , Georg Duda (1) , Bettina Willie (1) , Wolfgang Wagermaier (2)

1 Charité - UniversitätsMedizin = Charité - University Hospital [Berlin]
2 Department of Biomaterials [Potsdam]
3 ESRF - European Synchrotron Radiation Facility
4 Department of Analytical Chemistry

Bone's mineral properties, such as particle thickness and degree of alignment have been associated with bone quality. Bone formation, remodeling, aging of the tissue and mineral homeostasis influence mineral particle properties leading to specific patterns across bone. Scanning small angle X-ray scattering (sSAXS) with synchrotron radiation is a powerful tool, which allows us to study bone's nanoscale mineral properties in a position-resolved way. We used sSAXS, fluorescence light microscopy and backscattered electron (BSE) imaging to study bone's mineral properties at the tibial midshaft of in vivo-loaded mice. By combining these techniques, we could detect local changes in mineral properties. Regions labeled with calcein fluorochrome have lower mean mineral thickness and degree of mineral alignment. We also observed thinner and less aligned mineral particles near blood vessels. We conclude that mineral properties (i) are altered by fluorochrome labeling and (ii) depend on the proximity to blood vessels.

Mots clés: Bone mineral; calcein; mice; synchrotron sSAXS

Domaines: Interactions cellulaires [q-bio.CB]; Matière Molle [cond-mat.soft]

Marta Aido, Michael Kerschnitzki, Rebecca Hoerth, Manfred Burghammer, Cédric Montero, et al.. Relationship between nanoscale mineral properties and calcein labeling in mineralizing bone surfaces. Connective Tissue Research, 2014, 55, pp.15-17. ⟨10.3109/03008207.2014.923869⟩. ⟨hal-01111212⟩