Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis.

Mathew S Box , Vincent Coustham (1) , Caroline Dean , Joshua S Mylne

1 IJPB - Institut Jean-Pierre Bourgin

Many experiments in modern plant molecular biology require the processing of large numbers of samples for a variety of applications from mutant screens to the analysis of natural variants. A severe bottleneck to many such analyses is the acquisition of good yields of high quality RNA suitable for use in sensitive downstream applications such as real time quantitative reverse-transcription-polymerase chain reaction (real time qRT-PCR). Although several commercial kits are available for high-throughput RNA extraction in 96-well format, only one non-kit method has been described in the literature using the commercial reagent TRIZOL.

Domaines: Sciences agricoles

Mathew S Box, Vincent Coustham, Caroline Dean, Joshua S Mylne. Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis.. Plant Methods, 2011, 7, pp.7. ⟨10.1186/1746-4811-7-7⟩. ⟨hal-01019127⟩