Isolation and characterization of 15 microsatellite loci in the poplar rust fungus, Melampsora larici-populina, and cross-amplification in related species

Benoît Barres (1) , Christian Cyril Dutech (2) , Axelle Andrieux (1) , Henri Caron (2) , Jean Pinon (1) , Pascal Frey (1)

1 IAM - Interactions Arbres-Microorganismes
2 BioGeCo - Biodiversité, Gènes et Ecosystèmes

We developed 15 microsatellite loci in the poplar rust fungus, Melampsora larici-populina, using two enrichment protocols. Polymorphism of each locus was assessed on a panel of 30 isolates, comprising three subpanels (world, regional and local scales). Thirteen loci were polymorphic with three to eight alleles detected. The 15 loci were also tested on five related Melampsora species, M. allii-populina, M. medusae f. sp. deltoidae, M. larici-tremulae, M. rostrupii and M. pinitorqua, and partial or global cross-amplification events were detected

Mots clés: MELAMPSORA LARICI-POPULINA; BASIDIOMYCETE; CROSS-SPECIES AMPLIFICATION; ENRICHED LIBRARY; MICROSATELLITE; FUNGI; MELAMPSORA ALLII-POPULINA; MELAMPSORA MEDUSAE; MELAMPSORA LARICI-TREMULAE; MELAMSPORA ROSTRUPII; MELAMPSORA PINITORQUA

Domaines: Ecologie, Environnement

Benoît Barres, Christian Cyril Dutech, Axelle Andrieux, Henri Caron, Jean Pinon, et al.. Isolation and characterization of 15 microsatellite loci in the poplar rust fungus, Melampsora larici-populina, and cross-amplification in related species. Molecular Ecology Notes, 2005, 6 (1), pp.60-64. ⟨10.1111/j.1471-8286.2005.01137.x⟩. ⟨hal-01005053⟩