SHOC1 and PTD form an XPF-ERCC1-like complexthat is required for formation of class I crossovers

Nicolas N. Macaisne (1) , Julien Vignard (2) , Raphaël R. Mercier (1)

1 IJPB - Institut Jean-Pierre Bourgin
2 ToxAlim-GS - Génotoxicité & Signalisation

Two distinct pathways for meiotic crossover formation coexist in most eukaryotes. The Arabidopsis SHOC1 protein is required forclass I crossovers and shows sequence similarity with the XPF endonuclease family. Active XPF endonucleases form a heterodimerwith ERCC1 proteins. Here, we show that PTD, an ERCC1-like protein, is required for class-I-interfering crossovers along withSHOC1, MSH4, MSH5, MER3 and MLH3. SHOC1 interacts with PTD in a two-hybrid assay, through its XPF-like nuclease-(HhH)2domain. We propose that a XPF-ERCC1-like heterodimer, represented by SHOC1 and PTD in Arabidopsis, involving Zip2 inSaccharomyces cerevisiae and C9orf84 in human, is required for formation of class I crossovers.

Mots clés: Crossover; Meiosis; Recombination; XPF; ERCC1

Domaines: Sciences agricoles

Nicolas N. Macaisne, Julien Vignard, Raphaël R. Mercier. SHOC1 and PTD form an XPF-ERCC1-like complexthat is required for formation of class I crossovers. Journal of Cell Science, 2011, 124 (Pt 16), pp.2687-2691. ⟨10.1242/jcs.088229⟩. ⟨hal-01000742⟩