Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR.

Sami Slimani , Audrey Robyns , Sophie Jarraud (1) , Maëlle Molmeret , Eric Dusserre , Céline Mazure , Jean Pierre Facon , Gérard Lina (2) , Jerome Etienne (1) , Christophe Ginevra (1)

1 I2V - Immunité infection vaccination
2 Centre National de Reference des Staphylocoques

A PMA (propidium monoazide) pretreatment protocol, in which PMA is applied directly to membrane filters, was developed for the PCR-based quantification (PMA-qPCR) of viable Legionella pneumophila. Using this method, the amplification of DNA from membrane-damaged L. pneumophila was strongly inhibited for samples containing a small number of dead bacteria.

Domaines: Sciences du Vivant [q-bio]

Sami Slimani, Audrey Robyns, Sophie Jarraud, Maëlle Molmeret, Eric Dusserre, et al.. Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR.. Journal of Microbiological Methods, 2012, 88 (2), pp.319-21. ⟨10.1016/j.mimet.2011.12.010⟩. ⟨hal-00965423⟩