A Physical Map of the 1-Gigabase Bread Wheat Chromosome 3B

Etienne Paux (1) , Pierre Sourdille (1) , Jerome J. Salse (1) , Cyrille C. Saintenac (1) , Frédéric Choulet (1) , Philippe Leroy (1) , Abraham A. Korol (2) , Monika M. Michalak (3) , Shahryar S. Kianian (3) , Wolfgang W. Spielmeyer (4) , Evans E. Lagudah (4) , Daryl D. Somers (5) , Andrzej A. Kilian , Michael M. Alaux (6) , Sonia S. Vautrin (7) , Helene H. Berges (7) , Kellye K. Eversole (8) , Rudi R. Appels (9) , Jan J. Safar (10) , Hana H. Simkova (10) , Jaroslav J. Dolezel (10) , Michel M. Bernard (1) , Catherine C. Feuillet (1)

1 GDEC - Génétique Diversité et Ecophysiologie des Céréales
2 Institute of Evolution
3 Department of Plant Sciences
4 Plant Industry
5 Cereal Research Centre
6 URGI - Unité de Recherche Génomique Info
7 CNRGV - Centre National de Ressources Génomiques Végétales
8 International Wheat Genome Sequencing Consortium
9 Centre for Comparative Genomics
10 Laboratory of Molecular Cytogenetics and Cytometry

As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence- based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17- billion- base- pair hexaploid bread wheat genome ( 2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome ( BAC)based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome- specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome- based approach.

Mots clés: GENOMIQUE; CHROMOSOME ARTIFICIEL; BACTERIE

Domaines: Biologie cellulaire

Etienne Paux, Pierre Sourdille, Jerome J. Salse, Cyrille C. Saintenac, Frédéric Choulet, et al.. A Physical Map of the 1-Gigabase Bread Wheat Chromosome 3B. Science, 2008, 322 (5898), pp.101-104. ⟨10.1126/science.1161847⟩. ⟨hal-00964135⟩