The relationship between post-prandial bile acid concentration, GLP-1, PYY and ghrelin.

Rachel E Roberts (1) , Clare Glicksman (2) , J Alaghband-Zadeh (2) , Roy Sherwood (2) , Naeem Akuji (2) , Carel Le Roux (3)

1 Medical School
2 Biochemistry
3 Imperial Weight Centre

Background: Gut hormones peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) play an integral role in appetite control and energy homeostasis. Entero-endocrine L-cells can be stimulated by nutrients and or bile acids to co-secrete PYY and GLP-1. The aim of this study was to determine the response of bile acids, PYY,GLP-1 and ghrelin after a test meal. Design: Twelve subjects with a BMI of 22.8 (0.52) kg/m2 (mean (SEM)) received a 400 kcal test meal after which blood samples were taken every 30 minutes from 0 to 180 minutes. PYY, GLP-1 and ghrelin were measured by radioimmunoassays. Fractionated bile acids were measured by HPLC-MSMS. Results: PYY positively correlated with glycochenodeoxycholic acid (GCDCA) (rs = 0.23, p = 0.03) and taurochenodeoxycholic acid (TCDCA) (rs = 0.26, p = 0.02). GLP-1 positively correlated with GCDCA (rs = 0.22, p = 0.047) and glycodeoxycholic acid (GDCA) (rs = 0.3, p = 0.005). Ghrelin negatively correlated with GDCA (rs = -0.45, p = < 0.0001), TCDCA (rs = -0.23, p = 0.034) and taurodeoxycholic acid (TDCA) (rs = -0.44, p = < 0.0001). Conclusion: PYY and GLP-1 responses correlated with chenodeoxycholic acid (CDCA) counterparts, whereas ghrelin negatively correlated with deoxycholic acid (DCA) counterparts. Specific bile acids may thus differentially affect entero-endocrine cells.

Mots clés: Medicine

Rachel E Roberts, Clare Glicksman, J Alaghband-Zadeh, Roy Sherwood, Naeem Akuji, et al.. The relationship between post-prandial bile acid concentration, GLP-1, PYY and ghrelin.. Clinical Endocrinology, 2010, 74 (1), pp.67. ⟨10.1111/j.1365-2265.2010.03886.x⟩. ⟨hal-00600463⟩