Nuclear envelope dynamics during plant cell division suggest common mechanisms between kingdoms

Katja Graumann , David E Evans (1)

1 Life Sciences

Behaviour of the NE (nuclear envelope) during open mitosis has been explored extensively in metazoans, but lack of native markers has limited similar investigations in plants. In this study, carried out using living synchronised tobacco BY-2 suspension cultures, the non-functional NE marker LBR-GFP and two native, functional NE proteins, AtSUN1 and AtSUN2, we provide evidence that the ER-retention theory for NE membranes is applicable in plants. We also observe two apparently unique plant features; location of the NE-membrane components in close proximity to chromatin throughout division and spatially distinct reformation of the NE commencing at the chromatin surface facing the spindle poles and concluding at the surface facing the cell plate. Mobility of the proteins was investigated in the interphase NE, during NE breakdown and reformation, in the spindle membranes and the cell plate. A role for AtSUN2 in nuclear envelope breakdown is suggested.

Mots clés: Life Sciences

Katja Graumann, David E Evans. Nuclear envelope dynamics during plant cell division suggest common mechanisms between kingdoms. Biochemical Journal, 2011, 435 (3), pp.661-667. ⟨10.1042/BJ20101769⟩. ⟨hal-00586467⟩