Validation of an optical surface plasmon resonance biosensor assay for screening (fluoro)quinolones in egg, fish, and poultry meat

Anne-Catherine Huet (1) , Caroline Charlier (2) , Stefan Weigel (3) , Samuel Godefroy (4) , Philippe Delahaut (2)

1 santés animale et humaine
2 santés animale et humaine
3 Institute of Food Safety
4 Food Research Division

A surface plasmon resonance biosensor immunoassay has been developed for multiresidue determination of 13 (fluoro)quinolone antibiotics in poultry meat, egg, and fish. The following performance characteristics were determined according to the guidelines laid down for screening assay validation in the European Decision 2002/657/EC: detection capability, specificity/selectivity, decision limit, repeatability, ruggedness, and stability. The detection capability estimated for norfloxacin, the reference fluoroquinolone, was below 0.5, 1, and 1.5 ng g-1 for poultry meat, egg and fish respectively. The screening assay proved specific and showed satisfactory sensitivity, below the MRL levels even though flumequine and oxolinic acid presented lower cross-reactivities. A wide range of non-MRL substances were also detected at concentrations below 10 ng g-1. Repeatability was good with both intra-assay and inter-assay coefficients of variation < 6 % and ruggedness was also demonstrated.

Mots clés: Life Sciences

Anne-Catherine Huet, Caroline Charlier, Stefan Weigel, Samuel Godefroy, Philippe Delahaut. Validation of an optical surface plasmon resonance biosensor assay for screening (fluoro)quinolones in egg, fish, and poultry meat. Food Additives and Contaminants, 2009, 26 (10), pp.1341-1347. ⟨10.1080/02652030903013328⟩. ⟨hal-00573907⟩