Detection of bluetongue serotype 4 in mouflons () from Spain

Belén Rodríguez-Sánchez , Pedro J. Sánchez-Cordón , Verónica Molina , María A. Risalde , Ana Cristina Pérez De Diego , José C. Gómez-Villamandos , José M. Sánchez-Vizcaíno

Bluetongue serotype 4 (BTV4) has been detected for the first time in tissue samples from 2 mouflons () from the South of Spain, in a retrospective study. The samples included in this study had been fixed and paraffin-embedded for over a year prior to their analysis using a BTV group-specific and a BTV4 specific RT-PCR test. Lung and lymphatic nodes were found positive in both specimens. The amplified DNA was confirmed to be BTV4 by sequencing the RT-PCR products and comparing them with other sequences from GenBank. The combination of RNA extraction from paraffin-embedded samples and serotype-specific real-time RT-PCR assays provides the tools for the detection of BTV from samples stored for a long time. The results shown in this study set out the basis for a greater survey with fixed samples from different species of wild ruminants that the veterinary services have been collecting for years.

Mots clés: Bluetongue; Serotype-4; Mouflons; Wild ruminants; RT-PCR; Paraffin-embedded tissues

Belén Rodríguez-Sánchez, Pedro J. Sánchez-Cordón, Verónica Molina, María A. Risalde, Ana Cristina Pérez De Diego, et al.. Detection of bluetongue serotype 4 in mouflons () from Spain. Veterinary Microbiology, 2010, 141 (1-2), pp.164. ⟨10.1016/j.vetmic.2009.08.003⟩. ⟨hal-00560848⟩