Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation
Résumé
A new primer pair (delta12^delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers. 1 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.