Cell Trapping Utilizing Insulator-based Dielectrophoresis in The Open-Top Microchannels

Chun-Ping Jen (1) , Yao-Hung Huang (1) , Teng-Wen Chen (1)

1 Department of Mechanical Engineering,

The ability to manipulate or separate a biological small particle, such as a living cell and embryo, is fundamental needed to many biological and medical applications. The insulator-based dielectrophoresis (iDEP) trapping is composed of conductless tetragon structures in micro-chip. In this study, a lower conductive material of photoresist was adopted as a structure in open-top microchannel instead of a metallic wire to squeeze the electric field in a conducting solution, therefore, creating a high field gradient with a local maximum. The microchip with the open-top microchannels was designed and fabricated herein. The insulator-based DEP trapping microchip with the open-top microchannels was designed and fabricated in this work. The cells trapped by DEP force could be further treated or cultured in the open-top microchannel ; however, those trapped in the microchip with enclosed microchannels could not be proceeded easily.

Domaines: Autre [cs.OH]

Chun-Ping Jen, Yao-Hung Huang, Teng-Wen Chen. Cell Trapping Utilizing Insulator-based Dielectrophoresis in The Open-Top Microchannels. DTIP 2008, Apr 2008, Nice, France. pp.289-291. ⟨hal-00277727⟩