Skip to Main content Skip to Navigation
Journal articles

Biocidal Properties of a Glycosylated Surface: Sophorolipids on Au(111)

Abstract : Classical antibacterial surfaces usually involve antiadhesive and/or biocidal strategies. Glycosylated surfaces are usually used to prevent biofilm formation via antiadhesive mechanisms. We report here the first example of a glycosylated surface with biocidal properties created by the covalent grafting of sophorolipids (a sophorose unit linked by a glycosidic bond to an oleic acid) through a self-assembled monolayer (SAM) of short aminothiols on gold (111) surfaces. The biocidal effect of such surfaces on Gram+ bacteria was assessed by a wide combination of techniques including microscopy observations, fluorescent staining, and bacterial growth tests. About 50% of the bacteria are killed via alteration of the cell envelope. In addition, the roles of the sophorose unit and aliphatic chain configuration are highlighted by the lack of activity of substrates modified, respectively, with sophorose-free oleic acid and sophorolipid-derivative having a saturated aliphatic chain. This system demonstrates thus the direct implication of a carbohydrate in the destabilization and disruption of the bacterial cell envelope.
Document type :
Journal articles
Complete list of metadatas

Cited literature [12 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01275050
Contributor : Gestionnaire Hal-Upmc <>
Submitted on : Friday, February 3, 2017 - 10:29:36 AM
Last modification on : Tuesday, November 17, 2020 - 2:04:02 PM
Long-term archiving on: : Friday, May 5, 2017 - 12:09:18 PM

File

10.1021-acsami.5b05090.pdf
Files produced by the author(s)

Identifiers

Citation

Claire Valotteau, Christophe Calers, Sandra Casale, Jan Berton, Christian V. Stevens, et al.. Biocidal Properties of a Glycosylated Surface: Sophorolipids on Au(111). ACS Applied Materials & Interfaces, Washington, D.C. : American Chemical Society, 2015, 7 (32), pp.18086-18095. ⟨10.1021/acsami.5b05090⟩. ⟨hal-01275050⟩

Share

Metrics

Record views

1069

Files downloads

574