Strong pollen limitation in genetically uniform hybrid chestnut orchards despite proximity to chestnut forests

Clément Larue (1, 2) , Rémy J Petit (1)

1 BioGeCo - Biodiversité, Gènes & Communautés
2 Invenio

Key message In arboriculture, the number and diversity of pollen donors can have a major impact on fruit production. We studied pollination insurance in hybrid chestnut orchards ( C. sativa × C. crenata ) provided by nearby wild European chestnuts ( C. sativa ) in southwestern France. Most fruits were sired by hybrid pollenizers rather than by wild chestnuts. When these hybrid pollenizers were too scarce, a frequent situation, pollen produced by wild chestnut trees did not compensate for the lack of compatible pollen and fertilization rates and fruit production collapsed. Context The demand for chestnuts has been increasing in recent years in many European countries, but fruit production is not sufficient to meet this demand. Improving pollination service in chestnut orchards could increase fruit production. Aims Investigate pollination service in chestnut orchards. Evaluate the contribution to pollination of trees growing in chestnut woods and forests. Methods We investigated five orchards planted with hybrid chestnuts ( C. sativa × C. crenata ) cultivars in southwestern France. We combined fruit set data, which provide information about pollination rate, with genetic data, which provide information about pollen origin. We used this information to estimate the contribution of nearby C. sativa forest stands to the pollination of each orchard. Results Pollination rates vary considerably, being fivefold higher in orchards comprising numerous pollen donors than in monovarietal orchards. Because of asymmetric hybridization barriers between hybrid and purebred cultivars, the surrounding chestnut forests provide very limited pollination insurance: less than 14% of the flowers in these monovarietal orchards had been pollinated by forest trees. Conclusion Because chestnut orchards are now increasingly relying on hybrid cultivars, surrounding wild European chestnut trees are no longer a reliable pollen source. To achieve maximal fruit set, efforts must therefore concentrate on orchard design, which should include enough cultivar diversity.

Mots clés: Fagaceae; Castanea sativa; Castanea crenata; Castanea mollissima; Eurojapanese hybrids; Pollination; Pollenizers; Paternity analysis; Genetic structure analysis; Fruit set Handling editor: Marjana Westergren

Domaines: Sciences de l'environnement

Clément Larue, Rémy J Petit. Strong pollen limitation in genetically uniform hybrid chestnut orchards despite proximity to chestnut forests. Annals of Forest Science, 2023, 80 (1), pp.37. ⟨10.1186/s13595-023-01188-6⟩. ⟨hal-04286789⟩